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Summary: The protein encoded by this gene is a member of the granzyme family. Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free Abcam catalog: ab215967 Raji cells were subjected to SDS PAGE followed by western blot with 10856-1-AP (H2AFX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. View All Images (12) FC experiment of HepG2 using 10856-1-AP 1X10^6 HepG2 cells were stained with 0.2ug Histone H2A.X antibody (10856-1-AP, red) and control antibody (blue). While methods such as western blots and immunohistochemistry are widely used but difficult to validate to regulatory standards, the ELISA method is the most quantifiable and easiest to validate.

Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (1 mg/mL).

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The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO (control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO(control),then cells were lysed in RIPA buffer with sonication.0.2 … Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells. In ataxia-telangiectasia cells lacking ATM kinase activity, gamma-H2AX was scarcely detectable in the mitotic chromosomes, suggesting involvement of ATM in M-phase phosphorylation of H2AX.

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Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - RIPA lysate (50 and 15 mcg) from HeLa cells that were either treated with etoposide (60 minutes) or mock treated. Flow Cytometry: gamma H2AX Phosphospecific Antibody [NB100-384] - Flow Cytometrical Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells.

H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family. The cellular localization is predicted to be nuclear. Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX).
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och ingår i mitosen H2AX, inkorporeras vid DNA-skada 143 Leucine-zippers, av gammaH2AX foci, kromosomala avvikelser och mikrokärnor) meriterande. Ytterligare meriter inkluderar vana med Western blot-analys och cellodling. av gammaH2AX foci, kromosomala avvikelser och mikrokärnor) meriterande. Ytterligare meriter inkluderar vana med Western blot-analys och cellodling.

gamma-H2AX Antibody A300-081A-T TDetection of mouse gamma-H2AX by western blot. Samples:Whole cell lysate (50 µg) from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody:Affinity purified rabbit anti-gamma-H2AX antibody A300-081A-T (lot A300-081A-T-21) used for WB at 1:1000. Detection:Chemiluminescence with an Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated by ATM and ATR MA1-2022 detects human and mouse phosphorylated H2AX.
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Application: Western Blot (WB) Immunocytochemistry (ICC) ELISA Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2021-04-10 · Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate using MA1-2022. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX Western blot analysis of Phospho-H2AX pSer140 (MA1-2022) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) Secondary Antibody.

Antibody:Affinity purified rabbit anti-gamma-H2AX antibody A300-081A-T (lot A300-081A-T-21) used for WB at 1:1000. Detection:Chemiluminescence with an Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes.
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Histone h2ax är en kritisk faktor för cellulärt skydd mot dna

Robust bands can be visualized after probing western blots with Rockland's array of antibodies. Western Blot Detection Cartridge transforms the SpectraMax i3x and SpectraMax Paradigm Multi-Mode Platforms into a western blot reader while sustaining the Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as γ-H2AX in response to double-strand DNA damage and apoptosis. Signal-enhancing Western blot block solution for the best chemiluminescent signals. Enhanced signal – greatly enhances Western blotting signals; Decreased 2018年12月18日將lysis buffer 300μl（內含protinase inhibitor）加入放置於4℃ 冰鎮之脊髓標本 Ependrof tube，並以Microson 機器打碎及sonication標本成均質 X Antibody (Ser 139) - Western Blotting. Click on image or enlarge button Western blot analysis of Histone H2A. The phosphorylated γ-H2A.X is also thought With H2O2 treatment, almost all cells demonstrated H(2)AX gamma at 1 h.

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H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

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MA1-2022 detects human and mouse phosphorylated H2AX. MA1-2022 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. Phospho gamma H2AX pSer139 Polyclonal Antibody for Western Blot IF ICC IHC P. Catalog Number: PA125001. Price: None. Category: Antibodies Secondary Detection Reagents.

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples.